SC/-Ade/-His/-Leu/-Trp/-Met Broth - Names and Identifiers
SC/-Ade/-His/-Leu/-Trp/-Met Broth - Physico-chemical Properties
Storage Condition | Room Temprature |
SC/-Ade/-His/-Leu/-Trp/-Met Broth - Introduction
SC/-Ade/-His/-Leu/-Trp/-Met Broth(SC/-Ade/-His/-Leu/-Trp/-Met medium) is a medium used for growth and selection of yeast (Saccharomyces cerevisiae). The following is a description of its nature, use, preparation and safety information:
Nature:
SC/-Ade/-His/-Leu/-Trp/-Met Broth is an inorganic salt-free basal medium supplemented with lactose and choline. It provides inorganic salts, nitrogen sources and carbon sources for yeast growth. Absence of specific amino acids Selective growth of specific strains can be achieved by selective addition of different selective supplements.
Use:
SC/-Ade/-His/-Leu/-Trp/-Met Broth is commonly used for yeast genome library screening, yeast mutation library screening, and yeast genetic experiments to locate and clone specific genes. This medium, through selective supplementation, limits the growth of only certain colonies containing the desired gene, facilitating the researchers to screen for the desired colonies.
Preparation Method:
to prepare SC/-Ade/-His/-Leu/-Trp/-Met Broth, first add an appropriate amount of SC/-Ura/-Trp paste medium into an appropriate amount of water, stir evenly, and then disinfect by boiling sterilization or filtration sterilization.
Safety Information:
SC medium is a basic inorganic salt medium, the composition is relatively simple, generally no major safety risks. However, in the process of preparation and handling, it is still necessary to pay attention to personal protective measures, such as wearing experimental gloves and goggles to avoid inhaling medium dust. At the same time, follow the basic safety operation procedures of the laboratory, properly handle the waste, to ensure the safety of the experiment.
Last Update:2024-04-09 21:11:58